vazyme ce design

  • Sales Proposal of Vazyme’s Solutions for Coronavirus

     · 1. About Vazyme 2. Vazyme Solution for Coronavirus Detection 3. Summary (Price / Lead Time / Production Capacity / Contact) 4. 2019-nCoV IgG/IgM Detection Kit (Colloidal Gold-Based) a. Product Information / Features / Pictures / Package b. Brochure c. Instruction for Use d. Certificates (FDA, CEIVD, ISO 13485, CFDA) e. Clinical Test Report

  • miRNA1stStrandcDN A SynthesisKit(bystem-loop)

     · sequence based on the miRNA sequence.Reverse transcription and qPCR primer design can also be performed using the primer design softwaremiRNADesign(freedownload vazyme),whichcan beusedtoobtainprimersequences bysimplyinputtingthe

  • ClonExpress®Ultra One Step Cloning Kit(

     ·  CE Design 1. PCR,5’3’(1520 bp)。2×ClonExpress ® Mix,50℃5-15 min,。

  • Vazyme Biotech Co.,Ltd

    Relying on self-established critical generic technology platform, Vazyme successively marched into various business area biological research, in vitro diagnosis (IVD), bio-pharmaceutical and so on. Moreover, our company is regarded as one of the few domestic R&D innovative enterprises that have capabilities of both independent and controllable upstream technology development and terminal

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    CE Design(,). 0KB 0. Vazyme-HumanGenomicDNAQuantificationandQCKit-DataAnalysisTemplate. 222KB 0. . Vazyme miRNA. 641KB 0. . Vazyme

  • The synergistic effect of NELL1 and adipose-derived stem

     · The primers were designed by CE Design (Vazyme, China). Then the cloned Nell1 fragment was inserted into the linearized vector pCDH-CMV-MCS-EF1-copGFP by ClonExpress II One Step Cloning Kit (Vazyme, China) to construct pCDH-Nell1-copGFP (lenti-Nell1) vector.

  • Multi-Fragment Assembly (ClonExpress MultiS)crm.vazyme

     · Multi-Fragment Assembly (ClonExpress MultiS) * Please enter the complete sequence of the cloning vector or the sequence near the cloning site (5' to 3', and the upstream and downstream sequences of the cloning site are at least 20 bp each) 0. * Select the

  • STAU1 selectively regulates the expression of inflammatory

     · CE Design 1.04 software (Vazyme Biotech Co., Ltd.). was employed to design primers for Hot Fusion . There was a gene-specific sequence and a pIRES-hrGFP-1a vector (part no. 240031 Agilent Technologies, Inc.) sequence (17–30 bp) in each primer.

  • Primer Design for ClonExpress Entrycrm.vazyme

     · Primer Design for ClonExpress Entry. Primer Design for ClonExpress Entry. Single-Fragment Cloning. Single-Fragment Cloning (ClonExpress II) Multi-Fragment Assembly. Multi-Fragment Assembly (ClonExpress MultiS) Single-Point Mutation. Generate CE Entry Amplification Primers Empty

  • ClonExpress II One Step Cloning Kit

     · ,, >95%. CE Design. ClonExpress®、 DNA ,。. ClonExpress®II , Exnase®II。.

  • ClonExpressIIOneStepCloningkitCellagen Tech

     · VazymeBiotechCo.,Ltd Order [email protected] Support [email protected] vazyme Forresearchuseonly,notforuseindiagnosticprocedures. M DL5,000Marker.

  • AceQSYBR qPCRMasterMix

     · Vazyme biotech co., ltd. Catalog#Q111 Introduction AceQSYBR ®qPCRMasterMix The Vazyme AceQ SYBR ® qPCR Master Mix utilizes a special performance-enhanced AceTaq DNA Polymerase (Vazyme, #P401) protected via a chemically- modified hot-start activation technique to perform SYBR Green I based quantitative PCR (qPCR).

  • SRSF6 regulates alternative splicing of genes involved in

     · Primer pairs used for Hot Fusion were designed by CE Design v1.04 (Vazyme Biotech Co., Ltd) with gene-specific sequences and they included a portion of vector pIRES-hrGFP-1a sequences, with a 17–30 bp overlap between primer pairs.

  • How to Design a PrimerFujian Agriculture and Forestry

     · Primer design for PCR- recombination enzyme system For recombination enzyme system primer design, in our example, we will use C112 (Vazyme) kit as a model. The basic PCR primers for recombination cloning consist of Recombination Sequence 15bp homologous sequence of the backbone at the upstream and downstream of the cloning site.

  • CE Design V1.03(

     · CE Design V1.03(ClonExpress Mut Express) CE Design V1.03 Windows XP VB 6.0 ,

  • An Efficient Virus-Induced Gene Silencing System for

     · The fragments were amplified with primers designed by the software of CE Design (Vazyme, Nanjing, China Supplementary Table S2). 4 The pTRV2 vector was then digested with restriction endonuclease BamHI and XhoI (TAKARA, Japan). 5 A Gel Extraction Kit D2500 (Omega Biotek) 5 was used to purify the PCR product and the digested pTRV2 vector.

  • Genes Free Full-Text Cloning and Functional Analysis

    We designed primers to amplify the coding region of 4CL and CCoAOMT using CE Design (Vazyme Biotech Co., Ltd, Nanjing, China) and tested the amplification products by 1% agarose gel electrophoresis. We constructed 4CL and CCoAOMT overexpression vectors by linearizing and ligating the pBI121 vector with each insert using a ClonExpresss ® II One

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

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     · 15,Vazyme,PCR。DH5α(Vazyme #C502),XL10(Vazyme #C503),Fast-T1(Vazyme #C505),。

  • Biosynthesis Genes Cf4CL and CfCCoAOMT in

     · using CE Design (Vazyme Biotech Co., Ltd, Nanjing, China) and tested the amplification products by 1% agarose gel electrophoresis. We constructed 4CL and CCoAOMT overexpression vectors by linearizing and ligating the pBI121 vector with each insert using a ClonExpresssfi II One Step Cloning Kit C112 (Vazyme Biotech Co., Ltd, Nanjing, China).

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

  • Single-Fragment Cloning (ClonExpress II)crm.vazyme

     · Primer Design for ClonExpress Entry. Single-Fragment Cloning. Single-Fragment Cloning (ClonExpress II) Multi-Fragment Assembly. Generate CE II Amplification Primers Empty . 5'--3' 5'--3' * Select the restriction enzyme cleavage site that borders the 3' end of the insert

  • and Functional Analysis of Lignin Biosynthesis Genes

     · and CCoAOMT using CE Design (Vazyme Biotech Co., Ltd, Nanjing, China) and tested the amplification products by 1% agarose gel electrophoresis. We constructed 4CL and CCoAOMT overexpression vectors by linearizing and ligating the pBI121 vector with each insert using a

  • Tm Calculatorcrm.vazyme

     · Tm Calculator. * Please enter the primer sequence (direction is from 5' to 3', non-ATCG characters will be automatically filtered) 0.

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

  • A multi‐functional genetic manipulation system and its use

     · All of the primers used in recombinant plasmid construction were automatically designed by CE Design Version 1.04 software (Vazyme). The counter-selection system AACC was constructed by replacing the AOX1 expression cassette in pPICZαA plasmid with an URA3 expression cassette, which was amplified form the genome of P. pastoris.

  • PbCSE1 promotes lignification during stone cell

     · CE Design V1.03 software was used to design the primer pairs containing Xba I and BamH I restriction sites among the pCAMBIA1300 vector. (VAZYME, Nanjing, China) to clone the target gene

  • ClonExpress II One Step Cloning Kit (

     · ClonExpress II One Step Cloning Kit ()(C112)ClonExpress® II One Step Cloning Kit、、,