clonexpress multis one step cloning kit

  • ClonExpress

     · ClonExpress.pdf,ClonExpressTM II One Step Cloning Kit Vazyme Biotech Co., Ltd w w /Web w . v /Tel a Vazyme biotech co., ltd. z y /Sales [email protected] m e . c

  • ClonExpress® Ultra One Step Cloning Kit

     · ClonExpress® Ultra One Step Cloning Kit C115-02 2-3 -DeepBio 5567.2 DeepBio ,,,

  • C113 ClonExpress MultiS One Step Cloning Kit

     · ClonExpressMultiSOneStepCloningKitisanewversioncloningkitbasedonClonExpressOneStepCloningtechnology.ExnaseMultiSandreactionbufferinthiskitareespeciallyoptimizedfortheassemblyofmultiplefragments.Withthehelpof thiskit,up tofivefragmentscan be orderly assembled in a single reaction. In addition, Exnase MultiS is compatible with the reaction systems of

  • Supplementary Material for

     · Phanta HS Master Mix (VP512-01) and ClonExpress MultiS One Step Cloning Kit (C113-02) from Vazyme Biotech. RFP-labeled Salmonella typhimurium was a gift from John Brumell (32). cDNAs for ZDHHC5, ZDHHC20, NOD2, and NOD1 were gifts from Zongping Xia. The NF-κB reporter pNiFty3-Luc (pnf3-lc2) was purchased from Invivogen. Latex beads 3.84 µm

  • ClonExpress II One Step Cloning Kit (

     · 50 rxn. . ¥1000.0. ClonExpress®II One Step Cloning Kit. . 、、,. . 50 bp-10 kb DNA . PCR .

  • Hieff Clone® Plus Multi One Step Cloning Kit

     · Hieff Clone® Plus Multi One Step Cloning Kit 10912ES()Yeasen,Yeasen。()Hieff Clone® Plus

  • Biosynthesis of a ganoderic acid in Saccharomyces

     · ClonExpress MultiS One Step Cloning Kit (Vazyme, Nanjing, China) to yield a CYP expression vector, pRS426-HXT7p-FBA1t. The ORF of CYP candidates was cloned into pRS426-HXT7p-FBA1t to yield a series of CYP gene carrying plasmids using primer pairs described in Table S1. Another helper plasmid pRS425-TEF1p-PGK1t was con-

  • Discovery of an ene-reductase for initiating flavone and

     · The assembly of multiple DNA fragments in plasmid construction was performed by using the ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech

  • Team NKU CHINA/Protocols2018.igem

     · ClonExpress MultiS One Step Cloning kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer supplied in this kit are especially optimized for multi-insertion seamless cloning (MultiS for short). With the help of this kit, sequential assembly of up to five insertions can be realized in

  • Integrated regulatory network in Pseudomonas syringae

     · The PCR products were inserted into the digested pET28a plasmids (BamHI) using ClonExpress MultiS One Step Cloning Kit (Vazyme, China) generating the pET28a-transcriptional regulator plasmid. The successfully constructed plasmids were transformed into E. coli BL21 (DE3) strain respectively. Subsequently, a single colony on the plate was

  • ClonExpress II One Step Cloning Kit_——

    ClonExpress II One Step Cloning Kit-——-ClonExpress®IIOneStepCloningKit 、、,50bp-10kbDNA

  • Clone Express® MultiS One Step Cloning Kit

     · ClonExpress MultiS,bufferExnase MultiS。. ,。. ,Exnase MultiS、PCR。. PCRPCR

  • 5minTM TA/Blunt-Zero Cloning Kit(

     · ClonExpress MultiS One Step Cloning Kit ()(C113) ¥520 TUNEL BrightGreen Apoptosis Detection Kit()(A112) ¥1780

  • The Royal Society of Chemistry

     · ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech Co., Ltd). 20 μL reaction mixture containing 4 μL 5×CE MultiS Buffer, 2 μL Exnase MultiS, ~50 ng linear pET28a plasmid (pre- digested with NdeI/XhoI), ~20 ng fragment 1 (~ 900 bp), ~10 ng fragment 2 (~ 280 bp) and

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

  • ClonExpress MultiS One Step Cloning Kit

     · ClonExpress® MultiS One Step Cloning Kit ISO 9001 2015 SYSTEMS 01 02 Contents 02/ 01/ ClonExpress®、DNA,

  • C113-01 ClonExpress® MultiS One Step

    , C113-01 ClonExpress® MultiS One Step Cloning Kit, C113-01 ClonExpress® MultiS One Step Cloning Kit,3 ,

  • Team NKU China/Protocols2016.igem

     · ClonExpress MultiS One Step Cloning kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer supplied in this kit are especially optimized for multi-insertion seamless cloning (MultiS for short). With the help of this kit, sequential assembly of up to five insertions can be realized in

  • ClonExpress MultiS One Step Cloning Kit C113

    ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are especially optimized for the assembly of multiple fragments. With the help of this kit, up to 5 fragments can be orderly assembled in a single reaction.

  • ClonExpress®Ultra One Step Cloning Kit(

     · ClonExpress ® Ultra One Step Cloning Kit,1-5,2×ClonExpress Mix,50℃5-15 min,。. . C115-01 (25 rxn) C115-02 (50 rxn) 2 ×ClonExpress ® Mix. 125 μl. 2 ×125 μl. pCE

  • In-situ generation of large numbers of genetic

     · Recombination was conducted using the ClonExpress MultiS One Step Cloning Kit (Vazyme, Nanjing, China). Golden Gate assembly protocols were designed using j5 software tool (Revision as of

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     · ClonExpress MultiS One Step Cloning Kit ()(C113 ) (Vazyme) ¥ TUNEL BrightGreen Apoptosis Detection Kit(

  • ClonExpress MultiS One Step Cloning Kit (

     · ClonExpress MultiS One Step Cloning Kit ()(C113)ClonExpress® MultiS One Step Cloning Kit、、,

  • ClonExpress II One Step Cloning KitCellagen Technology

    ClonExpress II One Step Cloning (Vazyme, #C112) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning

  • 5minTM TA/Blunt-Zero Cloning Kit(

     · ClonExpress MultiS One Step Cloning Kit ()(C113) ¥520 TUNEL BrightGreen Apoptosis Detection Kit()(A112) ¥1780

  • 【ClonExpressTM II One Step Cloning Kit】

     ·  1 ClonExpress TM II 2 λDNA 500 bp , ClonExpress TM II One Step Cloning Kit pUC 19 EcoRI HindIII 。 PCR 。M Marker III;1-24, 24 。

  • ClonExpress MultiS One Step Cloning KitAbsource

    ClonExpress MultiS One Step Cloning Kit. 63.00 €131.00 €. excl. VAT plus shipping. Size. Choose an option 10 RXNS 25 RXNS. Clear. ClonExpress MultiS One Step Cloning Kit quantity. Add to cart. Categories Cloning & Mutagenesis, Fast Cloning, Molecular Biology SKU C113.

  • A small cysteine‐rich protein from two kingdoms of

     · The SP(VmE02)-INF1 SP, SP(PR1)-GFP, and SP(VmE02)-GFP expression constructs were generated using ClonExpress MultiS One-Step Cloning Kit (Vazyme). For generation of VmE02 genecomplementation constructs, PVX- VmE02 vector was used as template to amplify the sequence, and the fragments were then ligated to pDL2- GFP vector (Zhou et al ., 2011

  • 5minTM TA/Blunt-Zero Cloning Kit(

     · ClonExpress MultiS One Step Cloning Kit ()(C113) ¥520 TUNEL BrightGreen Apoptosis Detection Kit()(A112) ¥1780

  • .pdf BOOK118

     · .pdf,® ClonExpress MultiS One Step Cloning Kit C113 Vazyme biotech co., ltd. Version 7.1 Contents 01/02 02/ 02 03/ 02 04/ 02 05/ 02 06/ 03 07/ 03

  • Cloning & Mutagenesis / Reagents and Kits / Molecular

    ClonExpress MultiS One Step Cloning Kit ClonExpress II One Step Cloning Kit ClonExpress Ultra One Step Cloning Kit . 5min Universal Ligation Mix (1 / 1) 1 BioLinks Lab Supplies. Nijenheim 1325. 3704VC Zeist. The Netherlands. Tel . Email [email protected]